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  • Rabbit Anti-tubulin Beta Polyclonal Antibody
    货号: abs125209
    产品说明书
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    货号-规格 货期 价格 数量
    abs125209-50ul 现货 ¥1500.00
    - +
    abs125209-100ul 1-2周 ¥2300.00
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    大包装询价
    概述
    宿主
    Rabbit
    特异性
    Beta tubulin
    反应种属
    Human;Mouse;Rat;
    预测反应种属
    Rabbit;
    应用
    WB=1:5000-20000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
    免疫原
    KLH conjugated synthetic peptide derived from human tubulin Beta:61-160/444 
    性能
    浓度
    1mg/ml
    纯化方法
    affinity purified by Protein A
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    存储溶液
    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    研究领域
    免疫学  神经生物学  细胞骨架  
    靶标
    背景说明
    Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa.
    Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.
    细胞定位
    细胞浆 
    UniProt
    Sample:
    Brain (mouse) Lysate at 40 ug
    Heart (mouse) Lysate at 40 ug
    Primary: Anti- beta tubulin(abs125209)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD
    Sample: Spleen (Mouse) Lysate at 40 ug
    Primary: Anti- Beta tubulin (abs125209) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD
    Sample:
    Heart (Mouse) Lysate at 40 ug
    MCF-7 Cell (Human) Lysate at 40 ug
    Primary: Anti-Beta tubulin (abs125209) at 1/300 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD
    Sample:
    Brain (Rat) Lysate at 40 ug
    Heart (Rat) Lysate at 40 ug
    Primary: Anti-Beta tubulin (abs125209) at 1/300 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
    Predicted band size: 55 kD
    Observed band size: 50 kD
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (Beta tubulin ) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
    Incubation: Anti-Tubulin beta Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
    • 实验方法 实验条件
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