Rabbit anti-NCAM1 Polyclonal Antibody

NCAM1抗体;CD56; NCAM; MSK39; NCAM1 / CD56

反应种属：

Human, Mouse, Rat

应用：

ELISA 、IF/ICC 、IHC-P 、WB

来源宿主：

Rabbit

类型：

Polyclonal Antibody

对比

货号-规格	货期	价格	数量
abs118208-50uL	1-2周	￥ 1500 .00

订购信息：

您可以从我们的授权经销商处购买absin产品或获得技术支持。

研究领域

Neuroscience

Development

Neuroscience

Cell Adhesion Proteins

Neural Signal Transduction

Stem Cells

Hematopoietic Progenitors

Hematopoietic Stem Cells

Human Lineage Negative

实验结果图

Western blot analysis of various lysates using NCAM1 / CD56 Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of lysates from SH-SY5Y cells using NCAM1 / CD56 Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NCAM1 / CD56 Rabbit pAb at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NCAM1 / CD56 Rabbit pAb at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunofluorescence analysis of rat brain cells using NCAM1 / CD56 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of SH-SY5Y cells using NCAM1 / CD56 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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