Rabbit anti-Goat IgG-HRP Antibody

Interaction between host NUDT14 and viral RL13 proteins was analyzed using GST pull-down experiments. GST-tagged NUDT14 was used as a bait protein, and c-Myc-tagged RL13 was used as a prey protein. The recovered products were detected with either (A) mouse anti-c-Myc monoclonal antibody or (B) goat anti-GST monoclonal antibody. Lane 1, c-Myc-tagged RL13 and GST-labeled NUDT14 proteins captured by the MagneGST particles. Lane 2, c-Myc-labeled protein expressed from pGBKT7-RL13 in vitro. Lane 3, (A) commercial c-Myc-positive control protein or (B) protein lysates of BL21 (DE3) transfected with the GST-NUDT14-expressing plasmid (pGEX-4T2-NUDT14). GST, glutathione S-transferase; NUDT14, nucleo-side diphosphate-linked moiety X (nudix)-type motif 14.

UTI suppresses the IkB-a/NF-kB signaling pathway in RAW264.7 cells