Rabbit anti-TMEM119 Recombinant Monoclonal Antibody(S-838-46)

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling TMEM119 antibody at 1/500 dilution (0.1 ug)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

ICC shows positive staining in SH-SY5Y cells. Anti-TMEM119 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

WB result of TMEM119 Rabbit mAb Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution Lane 1: SH-SY5Y whole cell lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa Observed MW: 55 kDa Exposure time: 60 s (This blot was developed with high sensitivity substrate)

WB result of TMEM119 Rabbit mAb Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution Lane 1: mouse brain lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa Observed MW: 55 kDa Exposure time: 60 s (This blot was developed with high sensitivity substrate)

WB result of TMEM119 Rabbit mAb Primary antibody: TMEM119 Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa Observed MW: 55 kDa Exposure time: 60 s (This blot was developed with high sensitivity substrate)