Rabbit anti-FoxP3 Polyclonal Antibody

The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (gre

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-FoxP3/PE Conjugated antibody Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE . Protocol The cells were fixed with 4% PFA (10min at room temp

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells