Rabbit anti-CytokeRatin 19 Recombiant Monoclonal Antibody(107-19)

Flow cytometric analysis of MCF7  cells labelling Cytokeratin 19 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody. 

IHC shows positive staining in paraffin-embedded human colon.

Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta.

Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach.

Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney.

Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

WB resuLt of Cytokeratin 19 Rabbit mAb                

Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution
Lane 1: Hela whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDa
Exposure time: 20s

WB resuLt of Cytokeratin 19 Rabbit mAb                

Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution
Lane 1: mouse kidney lysate 20 µg

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDa
Exposure time: 120 s

WB resuLt of Cytokeratin 19 Rabbit mAb                

Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution
Lane 1: rat kidney whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 41 kDa
Observed MW: 39 kDa
Exposure time: 120 s

ICC shows positive staining in MCF7 cells. Anti-Cytokeratin 19 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubuLin (red).