Rabbit anti-CD9 Recombinant Monoclonal Antibody(143-53)

IHC shows positive staining in paraffin-embedded human tonsil.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.               

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.               

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostate.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.     

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human human ovarian cancer.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human human lung squamous cancer.Anti-CD9 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                                                 

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer.Anti-CD9 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.     

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human bladder cancer.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.       

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control:IHC shows negative staining in paraffin-embedded human placenta.Red blood cells is negative.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                       

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

WB resuLt of CD9 Rabbit mAb                 

Primary antibody: CD9 Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: Hela whole cell lysate 20 µg
Lane 4: HCT 116 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg

Negative control:

Raji whole cell lysate;                                   

K562 whole cell lysate                       

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDa
Exposure time: 180s

Flow cytometric analysis of  Raji (left) / HCT 116 (right) cells labelling CD9 antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: Raji

CD9 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CD9 in 0.4mg MCF7 whole cell lysate.
Western blot was performed on the immunoprecipitate using CD9 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : MCF7 whole cell lysate 10µg (input)
Lane 2 : CD9 Rabbit mAb IP in MCF7 whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in MCF7 whole cell lysate
Predicted MW: 22 kDa
Observed MW: 22 kDa
Exposure time: 80s