Mouse anti-SOX2 Monoclonal Antibody(57CT23.3.4)

Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody(Cat. #abs100224). abs100224 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.

Western blot analysis of lysate from SOX2 protein, using SOX2 Antibody(Cat. #abs100224). abs100224 was diluted at 1:4000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.

Fluorescent image of A549 cell stained with SOX2 Antibody(Cat#abs100224/SG110310AA).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.SOX2 immunoreactivity is localized to Nucleus significantly.

Western blot analysis of SOX2 Antibody (Cat.#abs100224) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.

Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#abs100224). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat.#abs100224), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.