Mouse anti-S100B Monoclonal Antibody

(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjug

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP K

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the

25 ug total protein per lane of various lysates (see on figure) probed with S100B monoclonal antibody, unconjugated at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to th

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat Anti

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat An

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat An

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to th

The A375 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by second

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by c

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by c

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by con

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by con