首页 产品中心
  • MMP13 Antibody (C-term)

    • MMP13抗体;Collagenase 3, 3424-, Matrix metalloproteinase-13, MMP-13, MMP13,MMP13
    货号: abs110501
    产品说明书
    分享:
    货号-规格 货期 价格 数量
    abs110501-50ul 现货 ¥1500.00
    - +
    大包装询价
    概述
    描述

    Purified Rabbit Polyclonal Antibody (Pab)

    别名
    MMP13抗体;Collagenase 3, 3424-, Matrix metalloproteinase-13, MMP-13, MMP13,MMP13
    宿主
    Rabbit
    特异性
    This MMP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the C-terminal region of human MMP13.
    反应种属
    Human
    应用
    WB~~1:1000
    IHC-P~~1:50~100
    分子量
    Predicted molecular weight: 54kD
    性能
    类型
    Polyclonal Antibody
    同种型
    Rabbit Ig
    储存/保存方法
    Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    存储溶液
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
    研究领域
    Cancer;Cardiovascular;Cell Biology;Signal Transduction
    靶标
    背景说明
    Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
    细胞定位
    Secreted, extracellular space, extracellular matrix. Secreted
    UniProt
    P45452
    参考文献
    参考文献
    Roy-Beaudry, M., et al., Arthritis Rheum. 48(10):2855-2864 (2003).
    Liacini, A., et al., Exp. Cell Res. 288(1):208-217 (2003).
    Hantke, B., et al., Biol. Chem. 384(8):1247-1251 (2003).
    Im, H.J., et al., J. Biol. Chem. 278(28):25386-25394 (2003).
    Tardif, G., et al., Osteoarthr. Cartil. 11(7):524-537 (2003).
    Anti-MMP13 Antibody (R310) at 1:500 dilution + MDA-MB-231 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    Western blot analysis of MMP13(arrow) using rabbit polyclonal MMP13 Antibody (Cat.#abs110501). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2) (Origene Technologies).
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
    • 实验方法 实验条件
      0张,还能上传 8 提交
          提示: 尊敬的客户您好,如果您对我们的产品有什么疑问或想要了解的,可以点击“我要提问”按钮填写您的疑问。
          提交不成功?请联系info@absin.cn。
          我要提问

      促销资讯 更多

        订购信息
        您可以从我们的授权经销商处购买absin产品或获得技术支持。若要查看您所在地区的经销商,请从以下的下拉列表中选择。
        GO
        • 0