Rabbit anti-MMP13 Polyclonal Antibody(C-term)

Anti-MMP13 Antibody (R310) at 1:500 dilution + MDA-MB-231 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of MMP13(arrow) using rabbit polyclonal MMP13 Antibody (Cat.#abs110501). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.