Goat anti-Mouse IgG-HRP Antibody

Effect of Compound 1 on the expression of the cell cycle—re—lated proteins in M DA—M B-231 cells

Effect of Compound 1 on the PI3K／AKT／mT0R signal pathway in M DA—M B-23 1 cells

Inhibitory effects of Compound 1 on the phosphorylat on levels of AKT and P70S6K in MDA-MI}23 1 cells at different time

A lower expression of AIF1, CXCL16 and Ki67 were detected in tumors derived from co-injection of RAW264.7 cells with downregulated AIF1 and Hepa1-6 cells, as compared with those from co-injection of control RAW264.7 cells and Hepa1-6 cells.

(A) Quantitative analysis of apoptotic HepG2 cells using an annexin V-FITC/PI flow cytometry assay at 48 h after different treatments. siGPC3 concentration: 0.1 μM; SF dose: 3 μM. Q1: normal viable cells; Q2: early-stage apoptotic cells; Q3: late-stage apoptotic cells; Q4: necrotic cells. (B) Cell cycle analysis of HepG2 cells at 48 h after the treatments. *p < 0.05 vs. controls; **p < 0.01 vs. PL/SCR; ΔΔp < 0.01 vs. PL/siGPC3.

Changes of mRNA and protein expression levels of Gadd45a, p21 in the kidney at 21 and 42 days of the experiment. a The relative mRNA expression levels of Gadd45a. b The western blot assay of Gadd45a. cThe relative protein expression levels of Gadd45a. d The relative mRNA expression levels of Cdc25C. e The western blot assay of p-Cdc25C. f The relative protein expression levels of p-Cdc25C. CG: Control group; GI:12 mg/kg group; GII: 24 mg/kg group; GIII: 48 mg/kg group. Data are presented with the mean ± standard deviation (n=8), *p < 0.05, compared with the control group; **p < 0.01, compared with the control group.

Pkm2 deficiency in IECs significantly reduced the pro-survival Wnt/β-catenin signaling. a Alteration of gene expression in Pkm2 −/−mouse IECs compared to that in fl/fl mouse IECs on day 3 post DSS treatment. b Selected genes that were altered in Pkm2 −/− mouse IECs compared to that in fl/fl mouse IECs on day 3 post DSS treatment. c Gene ontology gene analysis in fl/fl and Pkm2 −/− mouse IECs. In red were pathways enriched in Pkm2 −/− IECs. d WB analysis of levels of various proteins associated with Wnt/β-catenin signaling pathway.e Distribution and level of β-catenin and phosphorylated β-catenin (Y675) in Pkm2 −/− or fl/fl mouse IECs on day 3 post DSS treatment. f Under the stimulation of Wnt3a, cross-IP and cross-blot of PKM2 and β-catenin in IEC-6 cells using anti-β-catenin and anti-PKM2 antibodies, respectively. Immunoprecipitation using normal IgG served as controls. g β-Catenin levels in IEC-6 cells treated with Pkm2 siRNA or Pkm2 inhibitor/activator in the presence of 10% serum from colitic mice. Data are presented as the mean ±SEM from three independent experiments and p values are determined by the Student’s t test. ***p< 0.001; **p <0.01; *p<0.05; n.s. not significant

v-ATPase/mTORC1 signaling in mammary glands at lactation day 28. (A) Relative protein abundances of mTOR signaling and β-casein.The abundances of total mTOR, S6K, 4E-BP1, and β-casein were expressed in relation to that of β-actin. To quantify the specific phosphorylated protein, the calculated densitometric band intensities of the phosphorylated form were expressed in relation to that of the total protein on the same immunoblot, and the latter was determined after stripping and reprobing the membranes. Each data was then normalized to the CON group within an experiment.

The knockdown of FSIP1 attenuates the migration and invasiveness of breast cancer cells in vitro. A,Wound‐healing assay for T47Dand BT474 and wound closure was monitored at 0, 24, and 48 hours. ***P < .001. B, Left, representative images of invaded cells with Matrigel.Right, quantification of the numbers. ***P < .001. FSIP1, fibrous sheath interacting protein 1 [Color figure can be viewed at wileyonlinelibrary.com]

提取细胞蛋白样品进行wb实验，siGMPS可以显著抑制该蛋白表达，使用了爱必信的hrp二抗，所有条带均可曝光成功，表明该二抗性能良好