Rabbit anti-C-Peptide Recombinant Monoclonal Antibody(S-638-19)

WB result of Insulin Rabbit mAb Primary antibody: Insulin Rabbit mAb at 1/1000 dilution Lane 1: mouse skeletal muscle lysate 20 ug Lane 2: mouse pancreas lysate 20 ug Negative control: mouse skeletal muscle lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 9.4, 12 kDa Observed MW: 12, 13 kDa Exposure time: 33 s

IHC shows positive staining in paraffin-embedded human pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.