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Rabbit anti-ZO-1 Polyclonal AntibodyRabbit anti-ZO-1 Polyclonal AntibodyRabbit anti-ZO-1 Polyclonal AntibodyRabbit anti-ZO-1 Polyclonal Antibody
  • Rabbit anti-ZO-1 Polyclonal Antibody
  • Rabbit anti-ZO-1 Polyclonal Antibody
  • Rabbit anti-ZO-1 Polyclonal Antibody
  • Rabbit anti-ZO-1 Polyclonal Antibody
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  • Rabbit anti-ZO-1 Polyclonal Antibody

    货号-规格 货期 价格 数量
    abs122482-50uL 1-2周
    ¥0.00
    - +
    abs122482-100uL 1-2周
    ¥0.00
    - +
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    确定
    Sample: Lane 1: Huvec (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Primary: Anti-ZO-1 at 1/1000 dilution Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 220 kD Observed band size: 220 kD
    Blank control: 293T Cells(blue). Primary Antibody: Rabbit Anti-ZO-1/AF647 Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. Protocol The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
    Black line : Positive blank control (293T); Negative blank control (HL60) Green line : Primary Antibody (Rabbit Anti-ZO-1 antibody) Orange line:Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647) 293T(Positive)and HL60(Negative control)cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
    Independently Validated Antibody, image provided by Science Direct, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (abs122482) at 1:250 followed by conjugation to the secondary antibody.
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    • 能做WB的抗体就能做IHC,ELISA等实验吗?
    • 不一定,WB抗体识别的的蛋白经过加热变性之后都变成线性的结构 ,而IP抗体识别的是生理状态下的蛋白质,识别的位点可能被埋在蛋白质内部,也就是抗体识别的可能是内部构象表位,免疫荧光和免疫组化中需要进行固定,固定是为了尽量让细胞的形态结构维持和原有的一致。这种化学物质的固定使蛋白质变性凝固,与天然状态下的蛋白质有了一定的区别,但是又不同WB中加热变性变成了线性的结构。 所以购买的抗体具体应用于什么实验,要参考抗体说明书。
    • 一抗为什么会有预测种属?
    • 预测种属是理论上推测,不做质保,如果您的指标抗体比较难寻,可以选择尝试。
    • 选择一抗应该关注那些信息?
    • 目的蛋白的信息、抗体应用的实验类型、实验样本的种属信息、单抗和多抗的选择,如果您在选择抗体时不确定,可以联系我们的销售人员协助。

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