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abs60178-F-100ug 1-2周
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Fig. 3. Cellular uptake of RLNPs. (A) Flow cytometry analysis of the cellular uptake of FAM-mRNA RLNPs in GL261 cells at 2, 4, and 12 h. (B) Quantification analysis of the MFI of FAM-mRNA RLNPs in GL261 cells at 2, 4, and 12 h (n = 3). (C) CLSM images showing the endosomal escape of FAM-mRNA RLNPs in GL261 cells over time. Scale bar is 20 μm. (D) Intracellular distribution of FAM-mRNA and Cy5-siRNA in GL261 cells. Scale bar is 20 μm. Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
来源于《Int J Pharm》 DOI:10.1016/j.ijpharm.2026.126917
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