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  • Recombinant Mouse LIF
  • 货号: abs00998
    产品说明书
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    货号-规格 货期 价格 数量
    abs00998-5ug 1-2周 ¥756.00
    - +
    abs00998-25ug 1-2周 ¥1890.00
    - +
    abs00998-1mg 1-2周 ¥19968.00
    - +
    大包装询价
    概述
    种属
    Murine
    来源
    Escherichia coli.
    形态
    Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.
    性能
    氨基酸序列
    SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
    纯度
    > 98 % by SDS-PAGE and HPLC analyses.
    内毒素水平
    Less than 1 EU/μg of rMuLIF as determined by LAL method.
    活性
    Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.
    溶解方法
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
    储存/保存方法
    This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
    靶标
    Accession
    Gene IDs
    参考文献
    参考文献
    1. Eswari S, Sai Kumar G, Sharma GT. 2012. Zygote: 1-11.
    2. Mathieu ME, Saucourt C, Mournetas V, et al. 2012. Stem Cell Rev, 8: 1-15.
    3. Thomson AJ, Pierart H, Meek S, et al. 2012. Cell Reprogram, 14: 112-22.
    4. Li HandGrumet M. 2007. Glia, 55: 24-35.
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