人血清白蛋白

Activation of ROCK1 mediated PMPs generation under the high glucose stimulation. (A) Platelets were treated with MTB (Control) or 25 mmoL/L glucose plus 5 m g/mL collagen (HG þ Collagen) for 30 min. ROCK1 and ROCK2 mRNA expression levels were measured by real-time PCR. Results represent the mean ± SD. ***P < 0.001. (BeD) Platelets were pretreated with the ROCK inhibitor Y-27632 (10 m moL/L) and then stimulated with or without 25 mmoL/L glucose plus 5 m g/mL collagen as described in the section of Materials and Methods. (B) The protein expression levels of ROCK1 were measured by Western blotting. The histograms represent the mean ± SD of the ROCK1 expression levels and are normalized to GAPDH. *P < 0.05. (C) ROCK1 activity in platelets was evaluated by measuring the p-MYPT1 substrate levels. The histograms indicate the fold change of p-MYPT1 normalized to MYPT1 shown as the mean ± SD. **P < 0.01, ***P < 0.001. (D) The effects of ROCK1 on PMPs release from platelets. PMPs were counted by flow cytometry and the results represent the mean ± SD. **P < 0.01, ***P< 0.001.

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