Absin ECL化学发光检测试剂盒产品详情

1. 基本属性

中文名称	ECL化学发光检测试剂盒
英文名字	ECL luminescence reagent
别名	ECL发光液；HRP化学发光底物
产品货号	abs920
产品规格	2*25ml，2*50ml，2*250ml
产品组份	A液；B液
保存建议	2-8℃避光保存6个月。
产品说明书

2. 产品描述

Western blot底物发光检测试剂可由标记于二抗上的辣根过氧化酶催化，产生化学发光反应，可以灵敏地检测出目的蛋白的存在。ECL底物化学发光检测试剂盒基于新一代增强型化学发光底物研制而成，并对成份做了优化。产品背景低，稳定性好。它由辣根过氧化物酶(HRP)催化发生化学反应，发出荧光，可对X光胶片曝光，也可直接进行luminometer检测或者荧光CCD扫描。

3. 操作步骤

1. 按常规Western blot操作，二抗孵育后，进行最后一次洗涤时，根据膜的大小，按每10 cm2 膜混合0.5ml溶液A和0.5ml溶液B混匀，配制成发光检测工作液。
2. 用平头镊子将膜取出，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，置于洁净保鲜膜（某些市售保鲜膜包裹印迹膜时可能会淬灭荧光，应选择高质量保鲜膜）上。用吸管将配制的发光检测液转移到蛋白膜上，使其均匀覆盖，室温孵育2分钟。
3. 用平头镊夹持蛋白膜，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，包裹于洁净保鲜膜内。轻轻赶出其间的气泡，固定在X片暗盒内。
4. 在暗室中取一张X片置于包裹的膜上，合上暗盒，曝光30秒至1分钟。立即显影定影，根据其曝光强度，缩短或延长下一张X片的曝光时间（对微弱信号，曝光时间可延长至数小时）。

4. 用法说明

1.ECL A液和B液在吸取过程中必须要更换枪头，A液和B液相互污染后会导致 A液或B液逐渐失效，影响后续的使用效果。
2.各溶液使用后，请盖紧瓶盖，以防失效。特别是B液，含有氧化剂，比较容易 被还原而失效。
3.ECL的荧光持续时间很长，但开始反应后的30分钟内荧光更强一些，随后荧光会逐渐减弱，因此请注意充分利用这荧光较强的30分钟进行压片。
4.ECL A液和B液均对人体有害，操作时请注意适当防护。 为了您的安全和健康，请穿实验服并戴一次性手套操作。

ECL化学发光检测试剂盒部分文献展示

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  Frontiers in Physiology. 2021 Nov 11 .

  影响因子： 4.134

• Capsaicin restores sodium iodine symporter-mediated radioiodine uptake through bypassing canonical TSH‒TSHR pathway in anaplastic thyroid carcinoma cells

  Shichen Xu, Xian Cheng, Jing Wu, Yunping Wang, Xiaowen Wang, Liying Wu, Huixin Yu, Jiandong Bao, Li Zhang

  Journal of Molecular Cell Biology. 2021 Nov 09 .

  影响因子： 6.216

• Knockdown of long non-coding RNA AGAP2-AS1 suppresses the proliferation and metastasis of glioma by targeting microRNA-497-5p

  Yi Sun,Yulong Shen,Xing LiORCID Icon

  Bioengineered. 2021 Oct 28 .

  影响因子： 3.269

• Potentilla discolor ameliorates LPS-induced inflammatory responses through suppressing NF-κB and AP-1 pathways

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  Biomedicine & Pharmacotherapy. 2021 Oct 19 .

  影响因子： 6.529

• Caffeine‐induced neurotoxicity mediated by Nrf2 pathway in PC12 cells and zebrafish larvae

  S Chian, Z Jiang, L Jiang, K Wang

  JOURNAL OF APPLIED TOXICOLOGY. 2021 Sep 29 .

  影响因子： 3.446

• Cancer-Derived Exosomal miR-651 as a Diagnostic Marker Restrains Cisplatin Resistance and Directly Targets ATG3 for Cervical Cancer

  Xiaofan Zhu, Ling Long, He Xiao,Xuan He

  diseases markers. 2021 Sep 19 .

  影响因子： 3.434

• Sophoraflavanone M, a prenylated flavonoid from Sophora flavescens Ait., suppresses pro-inflammatory mediators through both NF-κB and JNK/AP-1 signaling pathways in LPS-primed macrophages

  Yixin Han, Xiaoyu Zhang, Yuan Kang, Yuan Gao, Ximeng Li, Ruijuan Qi, Runlan Cai, Yun Qi

  European Journal of Pharmacology. 2021 Sep 15 .

  影响因子： 3.100

• Sphingosine-1-phosphate induces myocyte autophagy after myocardial infarction through mTOR inhibition

  Li-Guo Yang, Ai-Ling Wang, Lu Li, Hong Yang, Xi Jie, Zong-Feng Zhu, Xiao-Juan Zhang, Hui-Ping Zhao, Rui-Fang Chi, Bao Li, Fu-Zhong Qin, Jia-Pu Wang, Ke Wang

  European Journal of Pharmacology. 2021 Sep 15 .

  影响因子： 4.432

• Role of caveolin‑1 in chronic postsurgical pain in rats

  Sai-Sai, Huang Su, Cao Yi-Bin, Qin Cui, E. Lu, Shi-Ren Shen

  Experimental and Therapeutic Medicine. 2021 Sep 13 ; 5

  影响因子： 2.447

• The Disruption of the Endothelial Barrier Contributes to Acute Lung Injury Induced by Coxsackievirus A2 Infection in Mice

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  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2021 Sep 13 .

  影响因子： 5.923

• Overexpression of RAB34 Associates With Tumor Aggressiveness and Immune Infiltration in Glioma

  Peng Hou, Quan Wan, Qing Wang, Xuechao Wu, Xiaojie Lu

  Research Square. 2021 Aug 17 .

• Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model

  Wangquan Ji, Peiyu Zhu, Ruonan Liang, Liang Zhang, Yu Zhang, Yuexia Wang, Weiguo Zhang, Ling Tao, Shuaiyin Chen, Haiyan Yang, Yuefei Jin, Guangcai Duan

  Viruses. 2021 Aug 11 ; 8

  影响因子： 5.048

• Long non-coding RNA Gm11974 aggravates oxygen–glucose deprivation-induced injury via miR-122-5p/SEMA3A axis in ischaemic stroke

  Ling Yang, Lijuan Wang, Juan Wang, Ping Liu

  Metabolic Brain Disease . 2021 Aug 02 .

  影响因子： 2.638

• HMGB1介导中性粒细胞自噬调控1,3-β-葡聚糖所致肺部炎症的作用机制

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• PAQR3逆转非小细胞肺癌A549/DDP细胞顺铂耐药机制研究

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• Hepatotoxicity of copper sulfide nanoparticles towards hepatocyte spheroids using a novel multi-concave agarose chip method

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  Future Medicine. 2021 Jun 29 .

  影响因子： 4.018

• LncRNA CASC2 Alleviates Sepsis-induced Acute Lung Injury by Regulating the miR-152-3p/PDK4 Axis

  Lili Zhu,Dongwu Shi,Jianghong Cao,Lu Song

  Immunological Investigations. 2021 Jun 24 .

  影响因子： 2.385

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