- ECL发光液；ECL luminescence reagent；HRP化学发光底物
| 货号-规格 | 货期 | 价格 | 数量 |
| abs920-2*25ml | 现货 | ￥210.00 | - + |
| abs920-2*50ml | 现货 | ￥368.00 | - + |
| abs920-2*250ml | 现货 | ￥1155.00 | - + |
大包装询价| 产品描述 | ||
| 描述 | Western blot底物发光检测试剂可由标记于二抗上的辣根过氧化酶催化，产生化学发光反应，可以灵敏地检测出目的蛋白的存在。ECL底物化学发光检测试剂盒基于新一代增强型化学发光底物研制而成，并对成份做了优化。产品背景低，稳定性好。它由辣根过氧化物酶(HRP)催化发生化学反应，发出荧光，可对X光胶片曝光，也可直接进行luminometer检测或者荧光CCD扫描。 | |
| 产品组分 | A液；B液 | |
| 操作步骤 | 1. 按常规Western blot操作，二抗孵育后，进行最后一次洗涤时，根据膜的大小，按每10 cm2 膜混合0.5ml溶液A和0.5ml溶液B混匀，配制成发光检测工作液。 2. 用平头镊子将膜取出，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，置于洁净保鲜膜（某些市售保鲜膜包裹印迹膜时可能会淬灭荧光，应选择高质量保鲜膜）上。用吸管将配制的发光检测液转移到蛋白膜上，使其均匀覆盖，室温孵育2分钟。 3. 用平头镊夹持蛋白膜，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，包裹于洁净保鲜膜内。轻轻赶出其间的气泡，固定在X片暗盒内。 4. 在暗室中取一张X片置于包裹的膜上，合上暗盒，曝光30秒至1分钟。立即显影定影，根据其曝光强度，缩短或延长下一张X片的曝光时间（对微弱信号，曝光时间可延长至数小时）。 | |
| 操作说明 | 1.ECL A液和B液在吸取过程中必须要更换枪头，A液和B液相互污染后会导致 A液或B液逐渐失效，影响后续的使用效果。
2.各溶液使用后，请盖紧瓶盖，以防失效。特别是B液，含有氧化剂，比较容易 被还原而失效。 3.ECL的荧光持续时间很长，但开始反应后的30分钟内荧光更强一些，随后荧光会逐渐减弱，因此请注意充分利用这荧光较强的30分钟进行压片。 4.ECL A液和B液均对人体有害，操作时请注意适当防护。 为了您的安全和健康，请穿实验服并戴一次性手套操作。 | |
| 基本信息 | ||
| 别名 | ECL发光液；ECL luminescence reagent；HRP化学发光底物
| |
| 概述 | ||
| 储存/保存方法 | 2-8℃避光保存6个月。 | |
| Western blot analysis of Rock-1 expression in Hep G2 (A) and NIH/3T3 (B) whole cell lysates. | ||
| Western blot analysis of NFATc3 isoform expression in Ramos whole cell lysate. | ||
| 温馨提示：本产品仅作科研实验使用，不支持临床等研究 |
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- 你们的ECL发光液要一分多钟才爆出结果，而其他品牌ECL发光液十几秒就爆出来了，是不是你们的产品灵敏度不够 ？
- 我们的ECL是防淬灭型的，可以维持在较长的时间内不淬灭，和市场上的快速曝光，但不能维持较长的时间的产品不同。各有优点，快速曝光是在短时间内快速燃烧底物，如果操作不快，很快就淬灭；防淬灭型的是缓慢燃烧底物，可以有充足的时间操作和应对突发事件。
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Certificate of Analysis(COA)
