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  • Recombinant SARS-CoV-2 Spike RBD (BA.2/Omicron) protein C-10His

    货号: abs06002
    产品说明书
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    货号-规格 货期 价格 数量
    abs06002-50ug 1-2周 ¥1500.00
    - +
    abs06002-100ug 1-2周 ¥2600.00
    - +
    abs06002-500ug 1-2周 ¥11000.00
    - +
    大包装询价
    概述
    种属
    SARS-CoV-2
    来源
    HEK 293
    分子量
    Detects a band of approximately 35kD (Predicted molecular weight: 26.5 kD)
    外观
    Lyophilized Powder
    形态
    Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
    性能
    序列
    Arg319-Lys537
    标签
    C-10His
    纯度
    >90% by SDS-PAGE
    内毒素水平
    Less than 1 EU per μg by the LAL method.
    标记
    unconjugated
    溶解方法
    Centrifuge tubes before opening.Reconstitute no more than 1 mg/mL according to the size in deionized water.
    储存/保存方法
    Within 1 month, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -80 °C as supplied. Avoid repeated freeze-thaw cycles.
    靶标
    背景说明
    Sars-cov-2 (2019-NCOV) infects human respiratory epithelial cells through binding to human ACE2 receptors. Spike protein is a large type I transmembrane protein containing two subunits S1 and S2.S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing cell surface receptors. S2 contains the basic elements required for membrane fusion. S protein plays a key role in inducing neutralizing antibody and T cell responses as well as protective immunity.
    Accession
    P0DTC2
    1μg(R: reducing conditions)
    Immobilized human ACE2-Fc at 2μg/ml (50μL/well) can bind to SARS-CoV-2 (BA.2/Omicron) RBD with a linear range of 0.17ng/ml to 10μg/ml.
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
    • 实验方法 实验条件
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      • 为什么理论分子量和实际分子量差距很大?
      • 一般蛋白理论分子量是根据氨基酸个数来计算的,氨基酸个数*110就是理论分子量的常规计算方法,但实际上蛋白还会经过很多修饰,比如糖基化、磷酸化等等,经过修饰的蛋白在电泳的时候就会表现出和理论分子量不太一样的分子量。

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