Recombinant SARS-CoV-2 3CLpro/3C-like protease Protein

SARS-CoV-2 3CLpro蛋白;3C-like protease、3CL Pro

SARS-CoV-2

E.coli

The protein has a predicted MW of 33.8 kDa same as Bis-Tris PAGE result.

Lyophilized from 0.22um filtered solution in 25mM HEPES, 2.5mM DTT, 10% glycerol (pH 7.5).

Ser1-Gln306

no tag

> 95% as determined by Bis-Tris PAGE

Less than 1 EU per μg by the LAL method.

unconjugated

Centrifuge tubes before opening.Reconstitute no more than 1 mg/mL according to the size in deionized water.

Within 1 month, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -80 °C as supplied. Avoid repeated freeze-thaw cycles.

3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.

YP_009725301.1