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  • Recombinant Tobacco Etch Virus rTEV Protease

    自产金标
    货号: abs05149
    产品说明书
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    货号-规格 货期 价格 数量
    abs05149-1KU 现货 ¥800.00
    - +
    abs05149-10KU 现货 ¥4800.00
    - +
    大包装询价
    概述
    种属
    Virus
    来源
    E.coli
    分子量
    28 kDa (Reducing)
    外观
    Liquid
    形态
    20mM Tris-HCl, pH8.0, 150mM NaCl, 1mM EDTA, 5mM DTT, 50%(v/v) Glycerol
    性能
    浓度
    1U/μL, 10U/μL
    纯度
    >98% SDS-PAGE & RP-HPLC
    内毒素水平
    <1EU/μg(LAL)
    标记
    unconjugated
    活性
    One unit of TEV protease cleaves ≥85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.
    储存/保存方法
    Within 1 month, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -80 °C as supplied. Avoid repeated freeze-thaw cycles.
    靶标
    背景说明
    The tobacco etch virus (TEV) protease is a useful tool for the removal of fusion tags from recombinant proteins. TEV protease has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin. Recombinant Tobacco Etch Virus Protease (rTEV) has (NIa) protease catalytic domain which corresponds to a molecular weight of 28 kDa. It is unique with high specificity and is active at low temperature. rTEV Protease has a 6*His-tag for easy removal from a reaction using nickel affinity resins and has been engineered to improve thermal stability and decrease autolysis.
    SDS-PAGE: 1μg(R: reducing conditions, N: non-reducing conditions).
    RP-HPLC
    One unit of TEV protease cleaves ≥85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.
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    • 实验方法 实验条件
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