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  • Rabbit anti-ZBTB7B (C-term) Polyclonal Antibody
  • Zinc finger and BTB domain-containing protein 7B, Krueppel-related zinc finger protein cKrox, hcKrox, T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 15, Zinc finger protein 67 homolog, Zfp-67, Zinc finger protein 857B, Zinc finger protein Th-POK, ZBTB7B, ZBTB15, ZFP67, ZNF857B;ZBTB7B;ZBTB15, ZFP67, ZNF857B
  • 货号: abs110587
    产品说明书
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    货号-规格 货期 价格 数量
    abs110587-50ul 1-2周 ¥1250.00
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    大包装询价
    概述
    描述
    Purified Rabbit Polyclonal Antibody (Pab)
    别名
    Zinc finger and BTB domain-containing protein 7B, Krueppel-related zinc finger protein cKrox, hcKrox, T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 15, Zinc finger protein 67 homolog, Zfp-67, Zinc finger protein 857B, Zinc finger protein Th-POK, ZBTB7B, ZBTB15, ZFP67, ZNF857B;ZBTB7B;ZBTB15, ZFP67, ZNF857B
    宿主
    Rabbit
    特异性
    This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-469 amino acids from the C-terminal region of human ZBTB7B.
    反应种属
    Human
    应用
    WB~~1:1000
    IF~~1:10~50
    性能
    类型
    Polyclonal Antibody
    同种型
    Rabbit Ig
    储存/保存方法
    Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    存储溶液
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
    研究领域
    Immunology
    靶标
    背景说明
    Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes (By similarity).
    细胞定位
    Nucleus.
    UniPort
    O15156
    参考文献
    参考文献
    Galera,P., Proc. Natl. Acad. Sci. U.S.A. 91 (20), 9372-9376 (1994)
    Widom,R.L., Gene 198 (1-2), 407-420 (1997)
    Heegaard,A.M., J. Bone Miner. Res. 12 (12), 2050-2060 (1997)
    Widom,R.L., Matrix Biol. 20 (7), 451-462 (2001)
    All lanes : Anti-ZBTB7B Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term)(Cat#abs110587).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.
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