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  • Rabbit anti-ZAP70 (Center) Polyclonal Antibody
  • Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK;ZAP70;SRK
  • 货号: abs113263
    产品说明书
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    货号-规格 货期 价格 数量
    abs113263-50ul 1-2周 ¥1250.00
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    大包装询价
    概述
    别名
    Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK;ZAP70;SRK
    宿主
    Rabbit
    特异性
    This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-282 amino acids from the Central region of human ZAP70.
    反应种属
    Human
    应用
    WB~~1:1000
    IHC-P~~1:50~100
    性能
    类型
    Polyclonal Antibody
    同种型
    Rabbit Ig
    储存/保存方法
    Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    存储溶液
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
    研究领域
    Signal Transduction;Immunology;Others
    靶标
    背景说明
    Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
    细胞定位
    Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity).
    UniPort
    参考文献
    参考文献
    Kuroyama, H., et al., Biochem. Biophys. Res. Commun. 315(4):935-941 (2004).
    Yang, Y., et al., Mol. Cell. Biol. 23(21):7667-7677 (2003).
    Kuhne, M.R., et al., J. Immunol. 171(2):860-866 (2003).
    Kremer, K.N., et al., J. Immunol. 171(1):360-367 (2003).
    Wiestner, A., et al., Blood 101(12):4944-4951 (2003).
    All lanes : Anti-ZAP70 Antibody (A267) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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