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  • Rabbit anti-ZAK Polyclonal Antibody
  • AZK;MLK7;mlklak;MLT;MLTK;MLTKalpha;MLTKbeta;MRK;pk;SFMMP;ZAK;AZK;MLK7;mlklak;MLT;MLTK;MRK;pk;SFMMP
  • 货号: abs117853
    产品说明书
    分享:
    货号-规格 货期 价格 数量
    abs117853-50ul 1-2周 ¥1250.00
    - +
    大包装询价
    概述
    别名
    AZK;MLK7;mlklak;MLT;MLTK;MLTKalpha;MLTKbeta;MRK;pk;SFMMP;ZAK;AZK;MLK7;mlklak;MLT;MLTK;MRK;pk;SFMMP
    宿主
    Rabbit
    反应种属
    Human;Mouse;Rat
    应用
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:20 - 1:50
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ZAK (NP_598407.1).
    阳性对照
    MCF-7,A-549,HepG2,Mouse lung,Rat brain,Mouse heart
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
    细胞定位
    Cytoplasm,Nucleus
    UniPort
    Western blot analysis of extracts of various cell lines, using ZAK antibody (abs117853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
    Immunohistochemistry of paraffin-embedded human colon carcinoma tissue using ZAK antibody at dilution of 1:100 (40x lens).
    Immunohistochemistry of paraffin-embedded mouse esophageal cancer tissue using ZAK antibody at dilution of 1:100 (40x lens).
    Immunofluorescence analysis of A549 cell using ZAK antibody. Blue: DAPI for nuclear staining.
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