概述 | ||
别名 | TMEM189抗体;KUA | |
宿主 | Rabbit | |
反应种属 | Human | |
应用 | WB 1:500 - 1:2000 IHC 1:50 - 1:100 | |
分子量 | Detects a band of approximately 31kD (Predicted molecular weight: 30kD/31kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human TMEM189 (NP_954580.1). | |
阳性对照 | MCF-7 | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | Co-transcription of this gene and the neighboring downstream gene (ubiquitin-conjugating enzyme E2 variant 1) generates a rare read-through transcript, which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. The protein encoded by this individual gene lacks a UEV1 domain but includes three transmembrane regions. Alternative splicing results in multiple transcript variants. | |
细胞定位 | Endoplasmic reticulum membrane,Multi-pass membrane protein | |
UniProt | ||
Western blot analysis of extracts of MCF-7 cells, using TMEM189 antibody (abs118451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s. | ||
Immunohistochemistry of paraffin-embedded human liver using TMEM189 antibody at dilution of 1:100 (40x lens). | ||
Immunohistochemistry of paraffin-embedded human stomach using TMEM189 antibody at dilution of 1:100 (40x lens). | ||
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- 实验方法 实验条件
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