概述 | ||
别名 | SCAMP1抗体;SCAMP,SCAMP37 | |
宿主 | Rabbit | |
反应种属 | Human;Mouse;Rat | |
应用 | WB 1:500 - 1:2000 | |
分子量 | Detects a band of approximately 38kD (Predicted molecular weight: 18kD/37kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SCAMP1 (NP_004857.4). | |
阳性对照 | U-87MG,MCF-7,22Rv1,HT-1080,Mouse brain,Rat brain | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | This gene product belongs to the SCAMP family of proteins, which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that these protein family members may function at the same site during vesicular transport rather than in separate pathways. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. | |
细胞定位 | Golgi apparatus,Multi-pass membrane protein,Recycling endosome membrane,trans-Golgi network membrane | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using SCAMP1 antibody (abs118628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 1s. | ||
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