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  • Rabbit anti-SART1 Polyclonal Antibody

    促销商品
    • SART1抗体,Ara1,HAF,HOMS1,SART1259,SNRNP110,Snu66,Ara1,HOMS1,SART1259,SNRNP110,Snu66
    货号: abs118573
    产品说明书
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    货号-规格 货期 价格 数量
    abs118573-50ul 1-2周 ¥1500.00 ¥1050.00
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    大包装询价
    概述
    别名
    SART1抗体;Ara1,HAF,HOMS1,SART1259,SNRNP110,Snu66,Ara1,HOMS1,SART1259,SNRNP110,Snu66
    宿主
    Rabbit
    反应种属
    Human;Mouse;Rat
    应用
    WB 1:500 - 1:2000
    分子量
    Detects a band of approximately 120kD (Predicted molecular weight: 90kD)
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).
    阳性对照
    HeLa,Jurkat,HepG2,Mouse liver,Mouse brain,Mouse testis,Rat brain
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.
    细胞定位
    Nucleus
    UniProt
    Western blot analysis of extracts of various cell lines, using SART1 antibody (abs118573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
    Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SART1 antibody (abs118573). Western blot was performed from the immunoprecipitate using SART1 antibody (abs118573) at a dilition of 1:1000.
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    • 实验方法 实验条件
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