Rabbit anti-PHF1 Polyclonal Antibody

PHF1抗体;hPCl1, MTF2 L2, MTF2L 2, MTF2L2, PCL 1, PCL1, PHD finger protein 1, PHD finger protein1, PHF 1, PHF 2, Phf1, PHF1_HUMAN, PHF2, Plc 1, Plc1, Polycomb like protein 1, Polycomb like protein1, Polycomb-like protein 1, Protein PHF1, T complex testis expressed 3, T complex testis expressed3, Tctex 3, Tctex3,

Rabbit

PHF1 Antibody detects endogenous levels of total PHF1.

Human;Mouse;Rat

Rabbit

WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Predicted molecular weight: 62KD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

A synthesized peptide derived from Human PHF1(Accession O43189), corresponding to amino acid residues E372-P397.

1mg/ml

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.

Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.

Cytoskeleton;Nucleus;