Rabbit anti-NUP133 Polyclonal Antibody
- NUP133抗体,hNUP133
货号: abs118616
货号-规格 | 货期 | 价格 | 数量 |
abs118616-50ul | 1-2周 | ¥1500.00 | - + |

概述 | ||
别名 | NUP133抗体;hNUP133 | |
宿主 | Rabbit | |
反应种属 | Human | |
应用 | WB 1:500 - 1:2000 IF 1:50 - 1:200 | |
分子量 | Detects a band of approximately 133kD (Predicted molecular weight: 128kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 730-930 of human NUP133 (NP_060700.2). | |
阳性对照 | A-375,LO2,HeLa,H460 | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. | |
细胞定位 | Chromosome,Nucleus,centromere,kinetochore,nuclear pore complex | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using NUP133 antibody (abs118616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s. | ||
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- 实验方法 实验条件
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