Rabbit anti-NR1H4 Polyclonal Antibody
- NR1H4抗体,BAR,FXR,HRR-1,HRR1,PFIC5,RIP14,BAR,FXR,HRR-1,HRR1,RIP14
货号: abs118409
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概述 | ||
别名 | NR1H4抗体;BAR,FXR,HRR-1,HRR1,PFIC5,RIP14,BAR,FXR,HRR-1,HRR1,RIP14 | |
宿主 | Rabbit | |
反应种属 | Human,Mouse | |
应用 | WB 1:500 - 1:2000 | |
分子量 | Detects a band of approximately 59kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 207-476 of human NR1H4 (NP_001193908.1). | |
阳性对照 | A-549,HepG2,HeLa,293T,SW480,Mouse liver,Rat kidney | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. | |
细胞定位 | Nucleus | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using NR1H4 antibody (abs118409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s. | ||
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- 实验方法 实验条件
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