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  • Rabbit anti-MGMT Polyclonal Antibody

    • MGMT抗体
    货号: abs133819
    产品说明书
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    货号-规格 货期 价格 数量
    abs133819-50ul 1-2周 ¥1500.00
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    大包装询价
    概述
    别名
    MGMT抗体
    宿主
    Rabbit
    反应种属
    Human,Rat
    应用
    WB 1:500 - 1:2000
    分子量
    Detects a band of approximately 22kD (Predicted molecular weight: 21kD)
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (XP_005252739.1).
    阳性对照
    Jurkat
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
    细胞定位
    Nucleus
    UniProt
    Western blot analysis of extracts of Jurkat cells, using MGMT antibody (abs133819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
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    • 实验方法 实验条件
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