Rabbit anti-MASTL Polyclonal Antibody

MASTL抗体;2700091H24Rik, C88295, FLJ14813, GREATWALL, Greatwall protein kinase, GW, GWL, GWL_HUMAN, hGWL, MAST-L, Mastl, MGC117975, Microtubule associated serine/threonine kinase like, Microtubule-associated serine/threonine-protein kinase-like, RP11 85G18.2, Serine/threonine-protein kinase greatwall, THC2,

Rabbit

MASTL Antibody detects endogenous levels of total MASTL.

Human;Mouse

Pig;Chicken;Sheep;Dog;Horse;Bovine;

WB 1:500~1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Predicted molecular weight: 97KD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

A synthesized peptide derived from Human MASTL(Accession Q96GX5), corresponding to amino acid residues L843-A865.

1mg/ml

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.

Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.

Cytoskeleton;Nucleus;