Rabbit anti-IRAK3 Polyclonal Antibody

IRAK3抗体;ASRT5, IL 1 receptor associated kinase M, IL-1 receptor-associated kinase M, IL1 receptor associated kinase M, Interleukin 1 receptor associated kinase 3, Interleukin 1 receptor associated kinase M, Interleukin-1 receptor-associated kinase 3, IRAK M, IRAK-3, IRAK-M, Irak3, IRAK3_HUMAN,

Rabbit

IRAK1 Antibody detects endogenous levels of total IRAK1.

Human;Rat

WB 1:500~1:1000, IHC: 1:50~1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Predicted molecular weight: 68KD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

A synthesized peptide derived from Human IRAK1(Accession P51617), corresponding to amino acid residues S371-G399.

1mg/ml

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity.Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation.

Cytosol;Endosome;Nucleus;Plasma Membrane;