Rabbit anti-IFIH1 Polyclonal Antibody

IFIH1抗体;CADM-140 autoantigen, Clinically amyopathic dermatomyositis autoantigen 140 kDa, DEAD/H (Asp Glu Ala Asp/His) box polypeptide, DEAD/H box polypeptide, Helicard, Helicase with 2 CARD domains, Hlcd, IDDM 19, IDDM19, IFIH 1, Ifih1, IFIH1_HUMAN, Interferon induced helicase C domain containing protein 1, interferon induced with helicase C domain 1, Interferon induced with helicase C domain protein 1, Interferon-induced helicase C domain-containing protein 1, Interferon-induced with helicase C domain protein 1, MDA 5, MDA-5, Melanoma differentiation associated protein 5, Melanoma differentiation-associated gene 5, Melanoma differentiation-associated protein 5, MGC133047, Murabutide down regulated protein, Murabutide down-regulated protein, RH 116, RH116, RIG I like receptor 2, RLR 2, RNA helicase DEAD box protein 116, RNA helicase-DEAD box protein 116,

Rabbit

IFIH1 Antibody detects endogenous levels of total IFIH1.

Human;Mouse

WB 1:500~1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Predicted molecular weight: 117kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

A synthesized peptide derived from Human IFIH1(Accession Q9BYX4), corresponding to amino acid residues R985-E1005.

1mg/ml

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.

Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.

Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta.Ubiquitinated by RNF125, leading to its degradation by the proteasome (PubMed:17460044). USP17/UPS17L2-dependent deubiquitination positively regulates the receptor (PubMed:20368735).During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.

Cytosol;Nucleus;