Rabbit anti-HNRNPL Polyclonal Antibody
- HNRNPL抗体,hnRNP-L,HNRPL,P/OKcl.14
货号: abs118485
货号-规格 | 货期 | 价格 | 数量 |
abs118485-50ul | 1-2周 | ¥1500.00 | - + |

概述 | ||
别名 | HNRNPL抗体;hnRNP-L,HNRPL,P/OKcl.14 | |
宿主 | Rabbit | |
反应种属 | Human;Mouse;Rat | |
应用 | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 | |
分子量 | Detects a band of approximately 64kD (Predicted molecular weight: 50kD/64kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 282-589 of human HNRNPL (NP_001524.2). | |
阳性对照 | U-87MG,HeLa,HepG2,A-549,HT-29,Mouse ovary,Rat lung,Rat brain | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. | |
细胞定位 | Cytoplasm,Nucleus,nucleoplasm | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using HNRNPL antibody (abs118485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s. | ||
Immunohistochemistry of paraffin-embedded human stomach using HNRNPL antibody at dilution of 1:100 (40x lens). | ||
Immunohistochemistry of paraffin-embedded rat kidney using HNRNPL antibody at dilution of 1:100 (40x lens). | ||
温馨提示:本产品仅作科研实验使用,不支持临床等研究 |
- 实验方法 实验条件
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