Rabbit anti-HGS Polyclonal Antibody

HGS抗体;Hepatocyte growth factor regulated tyrosine kinase substrate, Hepatocyte growth factor-regulated tyrosine kinase substrate, HGF-regulated tyrosine kinase substrate, HGNC:4897, HGS, HGS_HUMAN, Hrs, Human growth factor regulated tyrosine kinase substrate, Protein pp110, ZFYVE8,

Rabbit

HGS Antibody detects endogenous levels of total HGS.

Human;Mouse;Rat

WB 1:1000-3000, IHC 1:200, IF/ICC 1:200-1:500, ELISA(peptide) 1:20000-1:40000

Predicted molecular weight: 86kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

A synthesized peptide derived from Human HGS(Accession O14964), corresponding to amino acid residues I322-S345.

1mg/ml

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.Ubiquitinated by ITCH.

Cytosol;Endosome;Extracellular region or secreted;Lysosome;