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  • Rabbit anti-HERC3 Polyclonal Antibody

    • HERC3抗体
    货号: abs118571
    产品说明书
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    货号-规格 货期 价格 数量
    abs118571-50ul 1-2周 ¥1500.00
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    大包装询价
    概述
    别名
    HERC3抗体
    宿主
    Rabbit
    反应种属
    Human;Mouse
    应用
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    分子量
    Detects a band of approximately 117kD (Predicted molecular weight: 39kD/117kD)
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HERC3 (NP_055421.1).
    阳性对照
    SH-SY5Y,U-87MG,A-431,Mouse lung,Mouse spinal cord
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
    细胞定位
    Cytoplasm,Cytoplasmic vesicle
    UniProt
    Western blot analysis of extracts of various cell lines, using HERC3 antibody (abs118571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
    Immunohistochemistry of paraffin-embedded human prostate using HERC3 antibody at dilution of 1:100 (40x lens).
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
    • 实验方法 实验条件
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