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  • Rabbit Anti-GAPDH Polyclonal Antibody
    货号: abs124037
    产品说明书
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    货号-规格 货期 价格 数量
    abs124037-50ul 1-2周 ¥1500.00
    - +
    abs124037-100ul 1-2周 ¥2300.00
    - +
    大包装询价
    概述
    宿主
    Rabbit
    特异性
    GAPDH
    反应种属
    Human;Mouse;Rat;
    预测反应种属
    Rabbit;
    应用
    WB=1:2000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
    免疫原
    GAPDH protein of rabbit: 
    性能
    浓度
    1mg/ml
    纯化方法
    affinity purified by Protein A
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    存储溶液
    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    研究领域
    免疫学  
    靶标
    背景说明
    Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.
    细胞定位
    细胞核 细胞浆 细胞膜 
    UniPort
    Sample:
    Hela Cell (Human) Lysate at 40 ug
    MCF-7 Cell (Human) Lysate at 40 ug
    Primary: Anti- GAPDH (abs124037) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37 kD
    Observed band size: 37 kD
    Sample:
    Muscle (Rat) Lysate at 40 ug
    Heart (Rat) Lysate at 40 ug
    Primary: Anti- GAPDH (abs124037) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37 kD
    Observed band size: 37 kD
    Sample: Muscle (Mouse) Lysate at 40 ug
    Primary: Anti- GAPDH (abs124037) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37 kD
    Observed band size: 37 kD
    Sample:
    Thymus (Mouse) Lysate at 40 ug
    Primary: Anti- GAPDH(abs124037)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37 kD
    Observed band size: 37 kD
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining.
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
    Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
    Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
    Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
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