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  • Rabbit anti-EIF4A3 Polyclonal Antibody

    促销商品
    • EIF4A3抗体,DDX48,eIF4AIII,Fal1,MUK34,NMP265,NUK34,RCPS,DDX48,eIF4AIII,MUK34,NMP265,NUK34,RCPS
    货号: abs118866
    产品说明书
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    货号-规格 货期 价格 数量
    abs118866-50ul 1-2周 ¥1500.00 ¥1050.00
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    大包装询价
    概述
    别名
    EIF4A3抗体;DDX48,eIF4AIII,Fal1,MUK34,NMP265,NUK34,RCPS,DDX48,eIF4AIII,MUK34,NMP265,NUK34,RCPS
    宿主
    Rabbit
    反应种属
    Human;Mouse;Rat
    应用
    WB 1:1000 - 1:3000
    IHC 1:50 - 1:200
    分子量
    Detects a band of approximately 47kD (Predicted molecular weight: 46kD)
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human EIF4A3 (NP_055555.1).
    阳性对照
    A-431,Jurkat,HeLa,A-549,NIH/3T3,Mouse spleen,Rat kidney
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
    细胞定位
    Cytoplasm,Nucleus,Nucleus speckle
    UniProt
    Western blot analysis of extracts of various cell lines, using EIF4A3 antibody (abs118866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.
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    • 实验方法 实验条件
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