Rabbit anti-DHX38 Polyclonal Antibody
- DHX38抗体,DDX38; PRP16; PRPF16
货号: abs119022
货号-规格 | 货期 | 价格 | 数量 |
abs119022-50uL | 1-2周 | ¥1500.00 | - + |

概述 | ||
别名 | DHX38抗体;DDX38; PRP16; PRPF16 | |
宿主 | Rabbit | |
反应种属 | Human,Mouse,Rat | |
应用 | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 | |
分子量 | Detects a band of approximately 140kD (Predicted molecular weight: 60kD/140kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 1133-1227 of human DHX38 (NP_054722.2). | |
阳性对照 | HeLa,SKOV3,U-87MG,MCF-7,DU145,Mouse testis,Mouse thymus | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process. | |
细胞定位 | Nucleus | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using DHX38 antibody (abs119022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20s. | ||
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- 实验方法 实验条件
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