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  • Rabbit anti-DDX47 Polyclonal Antibody

    • DDX47抗体;E4-DBP,HQ0256,MSTP162,RRP3
    货号: abs133905
    产品说明书
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    货号-规格 货期 价格 数量
    abs133905-50ul 1-2周 ¥1500.00
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    大包装询价
    概述
    别名
    DDX47抗体;E4-DBP,HQ0256,MSTP162,RRP3
    宿主
    Rabbit
    反应种属
    Human;Mouse;Rat
    应用
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    分子量
    Detects a band of approximately 50kD (Predicted molecular weight: 45kD/50kD)
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human DDX47 (NP_057439.2).
    阳性对照
    U-87MG,SKOV3,LO2,HeLa,Mouse testis,Mouse lung,Rat testis
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
    细胞定位
    Nucleus,nucleolus
    UniProt
    Western blot analysis of extracts of various cell lines, using DDX47 antibody (abs133905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 90s.
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