概述 | ||
别名 | CYP46A1抗体;CP46,CYP46 | |
宿主 | Rabbit | |
反应种属 | Human;Mouse;Rat | |
应用 | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 | |
分子量 | Detects a band of approximately 57kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of human CYP46A1 (NP_006659.1). | |
阳性对照 | U-87MG,U-251MG,MCF-7,Mouse liver,Mouse brain,Rat liver | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. | |
细胞定位 | Endoplasmic reticulum membrane,Microsome membrane,Single-pass membrane protein | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using CYP46A1 antibody (abs118577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s. | ||
Immunohistochemistry of paraffin-embedded human prostate using CYP46A1 antibody at dilution of 1:100 (40x lens). | ||
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- 实验方法 实验条件
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