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  • Rabbit anti-CRHR1 Polyclonal Antibody

    • CRHR1抗体,CRF-R,CRF-R-1,CRF-R1,CRF1,CRFR-1,CRFR1,CRH-R-1,CRH-R1,CRHR,CRHR1L
    货号: abs118469
    产品说明书
    分享:
    货号-规格 货期 价格 数量
    abs118469-50ul 1-2周 ¥1500.00
    - +
    大包装询价
    概述
    别名
    CRHR1抗体;CRF-R,CRF-R-1,CRF-R1,CRF1,CRFR-1,CRFR1,CRH-R-1,CRH-R1,CRHR,CRHR1L
    宿主
    Rabbit
    反应种属
    Human;Mouse;Rat
    应用
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    分子量
    Detects a band of approximately 60kD
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 24-120 of human CRHR1 (NP_001138618.1).
    阳性对照
    U-87MG,SH-SY5Y,HT-29,HeLa,Mouse brain,Mouse liver,Mouse testis,Rat brain
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene.
    细胞定位
    Cell membrane,Endosome,Multi-pass membrane protein
    UniProt
    Western blot analysis of extracts of various cell lines, using CRHR1 antibody (abs118469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20s.
    Immunohistochemistry of paraffin-embedded rat brain tissue using CRHR1 antibody at dilution of 1:100 (40x lens).
    Immunohistochemistry of paraffin-embedded mouse brain tissue using CRHR1 antibody at dilution of 1:100 (40x lens).
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
    • 实验方法 实验条件
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