Claspin; claspin homolog (Xenopus laevis); Claspin homolog; CLSPN; CLSPN protein; CLSPN_HUMAN; hClaspin; Hu Claspin;
Claspin Antibody detects endogenous levels of total Claspin.
WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000
A synthesized peptide derived from human Claspin.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
Expression peaks at S/G2 phases of the cell cycle.
Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
|Western blot analysis Claspin using HUVEC whole cell lysates|
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