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  • Rabbit anti-CKMT2 Polyclonal Antibody
  • SMTCK
  • 货号: abs118309
    产品说明书
    分享:
    货号-规格 货期 价格 数量
    abs118309-50ul 1-2周 ¥1250.00
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    大包装询价
    概述
    别名
    SMTCK
    宿主
    Rabbit
    反应种属
    Human;Mouse;Rat
    应用
    WB 1:500 - 1:2000
    IHC 1:20 - 1:200
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 40-230 of human CKMT2 (NP_001816.2).
    阳性对照
    HeLa,Mouse skeletal muscle,Mouse heart,Mouse diaphragm,Rat skeletal muscle
    性能
    纯化方法
    Affinity purification
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.
    细胞定位
    Intermembrane side,Mitochondrion inner membrane,Peripheral membrane protein
    UniPort
    Western blot analysis of extracts of various cell lines, using CKMT2 antibody (abs118309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.
    Immunohistochemistry of paraffin-embedded rat heart tissue using CKMT2 antibody at dilution of 1:100 (40x lens).
    Immunohistochemistry of paraffin-embedded human esophagus tissue using CKMT2 antibody at dilution of 1:100 (40x lens).
    Immunohistochemistry of paraffin-embedded mouse heart tissue using CKMT2 antibody at dilution of 1:100 (40x lens).
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