Rabbit anti-CKAP5 Polyclonal Antibody
- CKAP5抗体,ch-TOG,CHTOG,MSPS,TOG,TOGp
货号: abs118553
货号-规格 | 货期 | 价格 | 数量 |
abs118553-50ul | 1-2周 | ¥1500.00 | - + |

概述 | ||
别名 | CKAP5抗体;ch-TOG,CHTOG,MSPS,TOG,TOGp | |
宿主 | Rabbit | |
反应种属 | Human;Mouse;Rat | |
应用 | WB 1:500 - 1:2000 | |
分子量 | Detects a band of approximately 250kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1). | |
阳性对照 | HeLa,Jurkat,HepG2,HT-29,Mouse brain,Mouse lung,Mouse liver,Rat brain | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. | |
细胞定位 | Cytoplasm,centrosome,cytoskeleton,microtubule organizing center,spindle pole | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using CKAP5 antibody (abs118553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s. | ||
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- 实验方法 实验条件
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