Rabbit anti-CAPN10 Polyclonal Antibody
- CAPN10抗体,CANP10,NIDDM1
货号: abs118696
货号-规格 | 货期 | 价格 | 数量 |
abs118696-50ul | 1-2周 | ¥1500.00 | - + |

概述 | ||
别名 | CAPN10抗体;CANP10,NIDDM1 | |
宿主 | Rabbit | |
反应种属 | Human | |
应用 | WB 1:500 - 1:2000 | |
分子量 | Detects a band of approximately 68kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. | |
免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CAPN10 (NP_075571.1). | |
阳性对照 | HeLa,A-375,HepG2,HT-29 | |
性能 | ||
纯化方法 | Affinity purification | |
类型 | Polyclonal Antibody | |
同种型 | IgG | |
储存/保存方法 | Store at -20℃. Avoid freeze / thaw cycles. | |
存储溶液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | |
靶标 | ||
背景说明 | Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. | |
UniProt | ||
Western blot analysis of extracts of various cell lines, using CAPN10 antibody (abs118696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s. | ||
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