Rabbit anti-BHLHB3 Polyclonal Antibody

BHLHB3抗体;Basic helix-loop-helix domain containing class B3, BHE41_HUMAN, bHLH protein DEC2, bHLHB3, bHLHe41, Class B basic helix loop helix protein 3, Class B basic helix-loop-helix protein 3, Class E basic helix-loop-helix protein 41, DEC2, Differentially expressed in chondrocytes protein 2, Enhancer of split and hairy related protein 1, Enhancer-of-split and hairy-related protein 1, hDEC2, Moderately similar to basic-helix loop helix protein M.musculus, SHARP-1,

Rabbit

BHLHB3 Antibody detects endogenous levels of total BHLHB3.

Human;Mouse;Rat

WB 1:500~1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Predicted molecular weight: 50kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

A synthesized peptide derived from Human BHLHE41(Accession Q9C0J9), corresponding to amino acid residues Y24-K46.

1mg/ml

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.

Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.

Nucleus;