Rabbit anti-β-Tubulin Polyclonal Antibody

Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- Beta Tubulin (abs120147) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Beta Tubulin (abs120147) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Blank control (blue line): Hela
Primary antibody: Rabbit Anti-Beta Tubulin antibody
Dilution: 1μg /10^6 cells;
Isotype Control antibody: Rabbit IgG .
Secondary antibody: Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.