Rabbit anti-RASSF1A Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for

Positive control: A549 cells (fixed with 70% ice-cold methanol overnight at 4℃,permeabilized with 0.1% PBS-Tween for 20 min at room temperature) Primary Antibody:Rabbit Anti-RASSF1A antibody green line;concentration: 1ug/Test Isotype Control Antibody: Rab

Sample: Lane 1: Thyroid gland (Mouse) Lysate at 40 ug Lane 2: Kidney (Mouse) Lysate at 40 ug Lane 3: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-RASSF1A at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band si

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (RASSF1A) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conju